An incident at a nightclub in Brighton last October led to the Malian midfielder, then a Brighton player, and a man in his 40s being investigated by police.
But it was confirmed on Wednesday that he will face no further action as the authorities continue their inquiries.
A spokesperson for Sussex Police said: “A man in his 40s from Brighton who was arrested on suspicion of sexual assault has had his conditional bail extended until July 4, 2022.
“A man in his 20s, also from Brighton, who was arrested on suspicion of sexual assault has been released with no further action to be taken.
“The investigation is ongoing and no additional information is available at this time.”
Bissouma, 25, signed for Antonio Conte’s Tottenham earlier this month in a deal worth £25million.